Rapid prototyping vaccine approach in mice against multi‐drug resistant Gram‐negative organisms from clinical isolates based on outer membrane vesicles

Hospital-acquired infections due to multi-drug resistant Gram-negative organisms (MDRGNO) pose a major threat global health. A vaccine preventing colonization and consecutive infection with MDRGNO could be particularly valuable, as therapeutic options become increasingly limited. Outer membrane vesicles (OMV) of Escherichia coli strain CFT073 well three strains that had caused severe in humans were administered intranasally mice, without cholera toxin an adjuvant. The humoral immune responses comparatively matched the sera patients, who suffered by respective bacterium. Additionally, systemic local toxicity was evaluated. Intranasal vaccination OMV elicit solid (total IgM IgG), specific for mice; decoration vital bacterial membranes antibodies comparable patients survived isolate. After intranasal mice no signs or observed. may open up rapid approach prevent and/or pathogenic MDRGNOs, especially outbreak setting within hospital. It also option have undergo elective interventions centers high risk certain common MDRGNO. Future studies need include challenge experiments phase I trials humans.